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Clostridial Proliferation and Intestinal Instability
George Tice, BVSc, MMedVet, MRCVS
The intention of this paper is to review the syndromes associated with clostridial overgrowth and proliferation as observed in Northern Europe particularly. Several reviews have already been published and salient points worthy of further study will be picked out.
The syndromes are:
All of these syndromes have been increasing in incidence in both the UK and in Holland (Fabri, 2000) and this has been associated with changes in the use of antibiotic growth promoters (AGPs).
This disease is well understood and is caused by Clostridium perfringens type A. The organism replicates readily in the intestinal tract where it produces alpha toxins which are believed to be responsible for the mucosal necrosis (Ficken et al, 1997). The chick is born with a sterile intestinal tract and for the first two weeks of life the oxygen content of the intestinal flora prevents the multiplication of clostridia which require anaerobic conditions (Mortimer, 2000). After the first two weeks clostridia begin to proliferate and clinical disease results if excessive multiplication takes place. An important characteristic of the disease is that subclinical disease which does not result in mortality but is associated with poor performance will be present and the disease can be graded according to severity (Prescott, 1979) and a subclinical form of NE described by Kaldhusdal et al (1992) has been characterised by focal ulceration in duodenum and jejunum.
Risk factors for the disease have been well described in literature, and these are:
Despite the identification of the different predisposing factors, no study has been performed that correctly calculates the relative risk of the different factors and no study on the relative importance of different environmental management strategies has been undertaken. Damage to the intestinal mucosa is also considered a predisposing factor in this disease (Truscott et al, 1977). Litter high in fiber content (Truscott et al, 1977) has been associated with NE, and concurrent coccidia infection (Shane et al, 1985; Baba et al, 1991) has been shown experimentally to predispose to the disease.
Cholangiohepatitis can cause excessive downgrading of between 3% and 5% of all chickens slaughtered, with severe economic consequences. But often, no disease has been reported on-farm. Clostridium perfringens is usually isolated in association with the disease, and antibiotic therapy in the young bird will control its development. Histologically the following is observed: a fibrosing hepatitis, proliferation of the bile ducts, multiple granulomas and compressed hepatocytes (due to proliferation of biliary ductules).
Published work is sparse. However, some work in Canada (Onderka et al, 1990) has experimentally reproduced the condition by either tying off the bile ducts or injecting Clostridium perfringens into the bile duct. The condition reproduced is virtually identical to that which is seen on the processing line. It therefore appears that the clostridia are again the culprit, either blocking the bile ducts or migrating into the liver to then upset liver function.
The assumption is made that the risk factors for the disease are the same as that for necrotic enteritis, but again this has not been confirmed with epidemiological studies.
The dysbacteriosis complex can be defined as the presence of a qualitatively and/or quantitatively abnormal flora in the small intestine and this causes a clinical disorder and/or malabsorption. It is generally seen after 21 days of age and is associated with wet faeces and a reduction in feed intake (Fabri, 2000). In Holland it is well-documented that the incidence of dysbacteriosis has increased since 1995 from 0.3% to 5.6% in 1999. The absence of AGPs, animal protein and animal fat appear to predispose farms to the disease. Certainly the incidence of dysbacteriosis in the UK since the removal of AGPs has increased significantly. Other predisposing factors may include non-specific stress, mycotoxins and systemic disease.
Bacterial overgrowth has been demonstrated in the intestines of birds affected with dysbacteriosis by the application of Terminal Restriction Fragment Length Polymorphism (T-RFLP) (Panneman et al, 2000), a method of monitoring intestinal microflora by DNA analysis. This method has demonstrated that the proximal intestine of normal birds has very low levels of bacteria, whereas birds affected with dysbacterioisis have substantially higher bacterial numbers. Clostridium spp. have been shown to contribute to this overgrowth. It has also been shown that affected birds, post-treatment with Tylan, had a similar bacterial population to normal healthy birds (Mortimer 2000).
Clostridial proliferation and intestinal instability are major threats to sustainable low-cost broiler production particularly in countries where large amounts of wheat are used. The removal of AGPs from part or all of the production cycle further compounds this threat, as does the imposition of long anticoccidial withdrawal periods. This emphasises the need to develop quantitative risk factors for the different syndromes along with medication regimes that maintain bird performance and intestinal stability most effectively.
Baba, E., Fuller, A.L., Gilbert, J.M., Thayer, S.G., McDougald, L.R. Effects of Eimeria brunetti infection and dietary zinc on experimental induction of necrotic enteritis in broiler chickens. Avian Diseases 1992; 36:59-62.
Branton, S.L., Lott, B.D., Deaton, J.W., Maslin, W.R., Austin, F.W., Pote, L.M., Keirs, R.W., Latour, M.A., Day, E.J. The effect of added complex carbohydrates or added dietary fiber on necrotic enteritis lesions in broiler chickens. Poultry Science 1997; 76:24-48.
Branton, S.L., Reece, F.N. Influence of a wheat diet on mortality of broiler chickens associated with necrotic enteritis. Poultry Science 1987; 66:1326-1330.
Elwinger, K., Engstrom, B., Berndtson, E., Fossum, O., Teglof, B. The effects of narasin on Clostridium perfringens in caeca and the occurrence of nectrotic enteritis in broiler chickens.Proc. XIX World Poultry Congr., 1992, Vol. 13. Amsterdam, The Netherlands, pp.580-584.
Fabri, T.H.F. Necrotic enteritis, Clostridial enteritis or dysbacteriosis? Proceedings of an Elanco Symposium, Cork, September 2000.
Ferket, P. Nutritional effects on enteric disorders. Enteric Disease Control Symposium, 39th Meeting of the American Association of Avian Pathologists, Louisville, Kentucky, 1996,pp.17-21.
Ficken, M.D., Wages, D.P. Necrotic enteritis. In: Diseases of Poultry 10th Edition. 1997. Iowa State Press, Ames, pp. 261-264.
Kaldhusdal, M., Hofshagen, M. Barley inclusion and avoparcin supplementation in broiler diets. 2. Clinical, pathological, and bacteriological findings in a mild form of necrotic enteritis. Poultry Science 1992; 71:1145-1153.
Kaldhusdal, M., Skjerve, E. Association between cereal contents in the diet and incidence of necrotic enteritis in broiler chickens in Norway. Preventive Veterinary Medicine 1996; 28:1-16.
Onderka, D.K., Langevin, C.C. and Hanson, J.A. Fibrosing cholehepatitis in broiler chickens induced by bile duct ligations or inoculation of Clostridium perfringens. Canadian Journal of Veterinary Research 1990; 54:285-290.
Mortimer, I. Tylan and necrotic enteritis. Proceedings of an Elanco Symposium, Cork, September 2000.
Panneman, H. Clostridial enteritis/dysbacteriosis, fast diagnosis by T-RFLP, a novel diagnostic tool. Proceedings of an Elanco Symposium, Montreal, 2000.
Prescott, J.F. The prevention of experimentally induced necrotic enteritis in chicken by avoparcin. Avian Diseases 24 (vol 4): 1073, 1979.
Shane, S.M., Gyimah, J.E., Harrington, K.S., Snider, T.G. Etiology and pathogenesis of necrotic enteritis. Veterinary Research Communications 1985; 9:269-287.
Smit, C. Viscosity of dietery fibre in relation to lipid disgestibility, 1997, Chapter PhD thesis, University of Wageningen.
Truscott, R.B., Al-Sheikhly, F. Reproduction and treatment of necrotic enteritis in broilers. American Journal of Veterinary Research 1977; 38(6):857-861.
Vissiennon Th., Kroeger, H., Kohler, T., Kliche, R. Effect of avilamycin, tylosin and ionophore anticoccidials on Clostridium perfringens enterotoxaemia in chickens. Berl. Munch. Tierarztl. Wschr., 2000; 113: 9-13.
Wicker, D.L., Isgrigg, W.N., Trammell, J.H., Davis, R.B. The control and prevention of necrotic enteritis in broilers with zinc bacitracin. Poultry Science 1977; 56:1229-1231.
George Tice, BVSc, MMedVet, MRCVS, is a Marketing Manager for Elanco Animal Health, United Kingdom. He began his career in animal health in 1989 as a veterinary surgeon in Bristol, England. After three years of work in large animal practice, Dr. Tice entered academia, serving as a member of the veterinary faculty at the Medical University of South Africa. In 1994 he joined Elanco as a veterinary adviser where he currently supports Elancos poultry team.